TISSUE PROCESSING & HEMATOXYLIN EOSIN STAINING
holaa :)
hoho . now aku nk cite psl praktikal yg kitowg wat ary tuh . kitowg wat tissue block sndiri , then wat staining . kitowg sume pakat amik BRAIN punye tissue . so sng masing2x nk tiru lab report t . hohoho :)
neh antare2x procedure nye :
neh suasana before praktikal tuh :)
neh brain tikus puteh oke ?
neh namenye grossing . utk identify appearance of the brain , colour and so on :)
neh tissue yg da dipotong and now kne bg masok dlm kaset utk diproses :)
neh namenye automated processor . utk process tissue td :)
neh utk meja grossing td :)
neh utk ltak kaset utk dimasukkan dlm automated processor td .
neh tyme tgh nk msokkn dlm bakul tuh . adoii .
neh bkaz utk H&E staining . utk kaler tissue properties such as nucleus and cytoplasm .
neh laa rupenye dlm automated processor .
neh computer yg terlekat kt ataz processor tuh .
oke . neh tissue block yg da di-WAX kn and disejukkan .
same mcm ataz :)
cool unit .
dispenser unit :) tmpt kuakan paraffin wax .
neh laa tissue block yg da perfectly perfect :)
neh namenye MICROTOME . ROTARY MICROTOME . utk hiris tissue block td utk jd snipis 10micrometer . [i guess ?]
neh saat2x tgh menghiris . ohh yeah , kak katy perry :)
afta dihiris2x , neh laa jdnye :) kne float kn tissue section td kt water bath n pick up gune slides :)
then , slides tuh akan distainkan dkt cneh yer :)
oke . afta diSTAININGkan , jd laa kaler2x :)
neh observation under microscope :) utk info kowg , yg kaler purple tuh nucleus , kaler pinkish tuh cytoplasm :)
peace no war :)
oraits sayang :) itu saje utk kaly neh . babai :) iloveyouu :)