TISSUE PROCESSING & HEMATOXYLIN EOSIN STAINING



holaa :)

hoho . now aku nk cite psl praktikal yg kitowg wat ary tuh . kitowg wat tissue block sndiri , then wat staining . kitowg sume pakat amik BRAIN punye tissue . so sng masing2x nk tiru lab report t . hohoho :)

neh antare2x procedure nye :


neh suasana before praktikal tuh :)


neh brain tikus puteh oke ?


neh namenye grossing . utk identify appearance of the brain , colour and so on :)


neh tissue yg da dipotong and now kne bg masok dlm kaset utk diproses :)


neh namenye automated processor . utk process tissue td :)


neh utk meja grossing td :)


neh utk ltak kaset utk dimasukkan dlm automated processor td .


neh tyme tgh nk msokkn dlm bakul tuh . adoii .


neh bkaz utk H&E staining . utk kaler tissue properties such as nucleus and cytoplasm .


neh laa rupenye dlm automated processor .


neh computer yg terlekat kt ataz processor tuh .


oke . neh tissue block yg da di-WAX kn and disejukkan .


same mcm ataz :)


cool unit .


dispenser unit :) tmpt kuakan paraffin wax .


neh laa tissue block yg da perfectly perfect :)


neh namenye MICROTOME . ROTARY MICROTOME . utk hiris tissue block td utk jd snipis 10micrometer . [i guess ?]


neh saat2x tgh menghiris . ohh yeah , kak katy perry :)



afta dihiris2x , neh laa jdnye :) kne float kn tissue section td kt water bath n pick up gune slides :)


then , slides tuh akan distainkan dkt cneh yer :)


oke . afta diSTAININGkan , jd laa kaler2x :)


neh observation under microscope :) utk info kowg , yg kaler purple tuh nucleus , kaler pinkish tuh cytoplasm :)


peace no war :)

oraits sayang :) itu saje utk kaly neh . babai :) iloveyouu :)


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